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TE15
TE15
規格:
價格:
編號:B165841
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
TE15
商品貨號
B165841
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with granular foci among the cellular swirls of Hassall's bodies.
The antibody binds specifically to granular foci among the cellular swirls of most Hassall's bodies and to stratum corneum of skin epidermis and foreskin.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human thymic epithelium (Hassall's bodies)
Cellular Products
immunoglobulin; monoclonal antibody; against human thymic epithelium (Hassall's bodies)
Comments
Animals were immunized with human thymic stroma.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with granular foci among the cellular swirls of Hassall's bodies.
It defines an antigen that is acquired between 14 and 16 weeks of fetal gestation, and marks the terminal stages of epidermal keratinocyte maturation.
The antibody binds specifically to granular foci among the cellular swirls of most Hassall's bodies and to stratum corneum of skin epidermis and foreskin.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%;
heat-inactivated fetal bovine serum, 20%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgM
Name of Depositor
BF Haynes
Deposited As
mouse (B cell); mouse (myeloma)
References

Lobach DF, et al. The human thymic microenvironment. Phenotypic characterization of Hassall's bodies with the use of monoclonal antibodies. J. Immunol. 134: 250-257, 1985. PubMed: 2578044

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