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TE3 [TE3A]
TE3 [TE3A]
規格:
價格:
編號:B165844
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
TE3 [TE3A]
商品貨號
B165844
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with an intracellular antigen expressed in cortical, but not medullary, thymic epithelium.
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human thymus (cortical thymic epithelium)
Cellular Products
immunoglobulin; monoclonal antibody; against human thymus (cortical thymic epithelium)
Comments
Animals were immunized with human thymic stroma.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with an intracellular antigen expressed in cortical, but not medullary, thymic epithelium.
All TE3+ cells in the thymus were found to express HLA and Ia antigens, and contain keratin.
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%;
heat-inactivated fetal bovine serum, 20%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2
Name of Depositor
BF Haynes
Deposited As
mouse (B cell); mouse (myeloma)
References

McFarland EJ, et al. The human thymic microenvironment: cortical thymic epithelium is an antigenically distinct region of the thymic microenvironment. J. Immunol. 133: 1241-1249, 1984. PubMed: 6205074

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