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TGP49
TGP49
規格:
價格:
編號:B165855
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
TGP49
商品貨號
B165855
Organism
Mus musculus, transgenic, mouse, transgenic
Cell Type
Epithelial,Epithelial-like
培養基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
pancreatic acinar cell tumor; carcinoma
Age
26 weeks
Gender
male
Strain
Tg(E1a-1-SV40E)Bri18
Applications
TGP49 is an epithelial-like cell line derived from a pancreatic tumor with acinar cell morphology that arose in an adult male transgenic mouse.
The mouse carried the pseudogene construct composed of elastase-1 promoter linked to the SV40 T antigen.
In culture, TGP49 cells lost acinar cell morphology and transiently produced somatostatin.
The cells do not secrete amylase or lipase.
Current stocks of TGP49 are negative for somatostatin and positive for trypsin.
Derivation
TGP49 is an epithelial-like cell line derived from a pancreatic tumor with acinar cell morphology that arose in an adult male transgenic mouse.
In culture, TGP49 cells lost acinar cell morphology and transiently produced somatostatin.
Clinical Data
TGP49 is an epithelial-like cell line derived from a pancreatic tumor with acinar cell morphology that arose in an adult male transgenic mouse.
male
Comments
TGP49 is an epithelial-like cell line derived from a pancreatic tumor with acinar cell morphology that arose in an adult male transgenic mouse.
The mouse carried the pseudogene construct composed of elastase-1 promoter linked to the SV40 T antigen.
In culture, TGP49 cells lost acinar cell morphology and transiently produced somatostatin.
Current stocks of TGP49 are negative for somatostatin and positive for trypsin. The cells do not secrete amylase or lipase. Morphologic and differentiation markers of this line appear to fluctuate from time to time in culture.
Complete Growth Medium
A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor
OS Pettengill, D Longnecker
Deposited As
mouse, transgenic
References

Pettengill OS, et al. Cell lines derived from pancreatic tumors of Tg(Ela-1-SV40E)Bri18 transgenic mice express somatostatin and T antigen. Carcinogenesis 15: 61-65, 1994. PubMed: 7904904

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