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THP-1
THP-1
規(guī)格:
價格:
編號:B165863
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
THP-1
商品貨號
B165863
Organism
Homo sapiens, human
Tissue
peripheral blood
培養(yǎng)基
Cell Type
monocyte
Product Format
frozen
Morphology
monocyte
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
acute monocytic leukemia
Age
1 year infant
Gender
male
Applications
These cells are suitable as a transfection host.
Storage Conditions
liquid nitrogen vapor phase
Images
TIB-202 Micrograph
Antigen Expression
HLA A2, A9, B5, DRw1, DRw2
Receptor Expression
complement (C3), expressed
Fc, expressed
Genes Expressed
lysozyme,HLA A2, A9, B5, DRw1, DRw2
Cellular Products
lysozyme
Comments
The cells are phagocytic (for both latex beads and sensitized erythrocytes) and lack surface and cytoplasmic immunoglobulin.
Monocytic differentiation can be induced with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2-4 x 105 viable cells/mL. Subculture when cell concentration reaches 8x105 cells/mL.Do not allow the cell concentration to exceed 1 x 106 cells/mL. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 13
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 8,9.3
TPOX: 8,11
vWA: 16
Name of Depositor
S Tsuchiya
Deposited As
Homo sapiens
References

Tsuchiya S, et al. Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester. Cancer Res. 42: 1530-1536, 1982. PubMed: 6949641

Skubitz KM, et al. Human granulocyte surface molecules identified by murine monoclonal antibodies. J. Immunol. 131: 1882-1888, 1983. PubMed: 6619543

Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019

Huang S, et al. Adenovirus interaction with distinct integrins mediates separate events in cell entry and gene delivery to hematopoietic cells. J. Virol. 70: 4502-4508, 1996. PubMed: 8676475

Clark RA, et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997. PubMed: 9151679

Hambleton J, et al. Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages. Proc. Natl. Acad. Sci. USA 93: 2774-2778, 1996. PubMed: 8610116

Hsu HY, et al. Inhibition of macrophage scavenger receptor activity by tumor necrosis factor-alpha is transcriptionally and post-transcriptionally regulated. J. Biol. Chem. 271: 7767-7773, 1996. PubMed: 8631819

Lucas M, Mazzone T. Cell surface proteoglycans modulate net synthesis and secretion of macrophage apolipoprotein E. J. Biol. Chem. 271: 13454-13460, 1996. PubMed: 8662812

Sando GN, et al. Induction of ceramide glucosyltransferase activity in cultured human keratinocytes. J. Biol. Chem. 271: 22044-22051, 1996. PubMed: 8703011

Ollivier V, et al. Elevated cyclic AMP inhibits NF-kappaB-mediated transcription in human monocytic cells and endothelial cells. J. Biol. Chem. 271: 20828-20835, 1996. PubMed: 8702838

Tsuchiya S, et al. Establishment and characterization of a human acute monocytic leukemia cell line (THP-1). Int. J. Cancer 26: 171-176, 1980. PubMed: 6970727

Cross References

Nucleotide (GenBank) : X70326 H.sapiens MacMarcks mRNA.

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