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TK6
TK6
規格:
價格:
編號:B165868
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
TK6
商品貨號
B165868
Organism
Homo sapiens, human
Tissue
spleen
培養基
Cell Type
lymphoblast
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
hereditary spherocytosis
Age
5 years
Gender
male
Applications
The cells are heterozygous at the thymidine kinase (TK) locus and can be used to quantitatively detect forward mutations at 3 loci (resistance to trifluorothymidine (tk locus)).
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
This line is derivative of the WIL-2 cell line.
Clinical Data
male
Comments
Resistance to thioguanine (hprt locus), and resistance to ouabain (Na/K ATPase).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Maintain the cell concentration between 2 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days (or as necessary to maintain the cell concentration between 2 X 105 and 1 X 106 cells/ml)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 12,13
D7S820: 9,11
THO1: 8,9.3
TPOX: 8,11
vWA: 17,20
Name of Depositor
Massachusetts Institute of Technology
Deposited As
Homo sapiens
U.S. Patent Number
References

Skopek TR, et al. Assay for mutagenesis in heterozygous diploid human lymphoblasts. US Patent 4,302,535 dated Nov 24 1981

Skopek TR, et al. Isolation of a human lymphoblastoid line heterozygous at the thymidine kinase locus: possibility for a rapid human cell mutation assay. Biochem. Biophys. Res. Commun. 84: 411-416, 1978. PubMed: 214074

Levy JA, et al. Human lymphoblastoid lines from lymph node and spleen. Cancer 22: 517-524, 1968. PubMed: 5673231

Dolganov GM, et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair. Mol. Cell. Biol. 16: 4832-4841, 1996. PubMed: 8756642

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