Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease
Lymphoma
Applications
The antibody reacts with a deteminant on the mouse T cell antigen receptor.
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse T cell receptor
Cellular Products
immunoglobulin; monoclonal antibody; against mouse T cell receptor
Comments
Animals were immunized with the Tk1 murine T cell lymphoma.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with a deteminant on the mouse T cell antigen receptor.
It recognizes a determinant encoded by the Vb7 (V beta 7) gene segment.
Complete Growth Medium
Culture Medium: RPMI medium 1640 with 2 mM L-glutamine, 1 mM sodium pyruvate and 50 uM 2-mercaptoethanol, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
