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TS1/22.1.1.13

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編號:B165882

品牌:Mingzhoubio

標準菌株

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DNA

RNA

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凍干粉

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產品名稱

TS1/22.1.1.13

商品貨號

B165882

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Tissue

spleen

培養基

Cell Type

hybridoma: B lymphocyte

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications

The depositor states that the cell line should not be maintained in culture continuously for greater than four months or antibody production may be lost.

Cloning and freezing of the cells after minimal passage (with selection of positive clones) will prevent loss of antibody producing cells.

Tested and found negative for ectromelia virus (mousepox).

Storage Conditions

liquid nitrogen vapor phase

Derivation

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against lymphocyte function antigen 1 (LFA-1) alpha subunit (CD11a)

Cellular Products

immunoglobulin; monoclonal antibody; against lymphocyte function antigen 1 (LFA-1) alpha subunit (CD11a)

Comments

Animals were immunized with human cytolytic T lymphocytes.

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

The antibody will inhibit HLA DR mediated T cell cytotoxicity.

The depositor states that the cell line should not be maintained in culture continuously for greater than four months or antibody production may be lost.

Cloning and freezing of the cells after minimal passage (with selection of positive clones) will prevent loss of antibody producing cells.

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml

Subcultivation Ratio: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Isotype

mouse IgG1

Name of Depositor

TA Springer

Deposited As

mouse (B cell); mouse (myeloma)

Passage History

Cloning and freezing of the cells after minimal passage (with selection of positive clones) will prevent loss of antibody producing cells.

References

Sanchez-Madrid F, et al. Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3. Proc. Natl. Acad. Sci. USA 79: 7489-7493, 1982. PubMed: 6984191

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