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當(dāng)前位置: 首頁 > ATCC代理 > U266B1 [U266] B165898
U266B1 [U266]
U266B1 [U266]
規(guī)格:
價格:
編號:B165898
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
U266B1 [U266]
商品貨號
B165898
Organism
Homo sapiens, human
Tissue
peripheral blood
培養(yǎng)基
Cell Type
B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
myeloma; plasmacytoma
Age
53 years adult
Gender
male
Applications
This cell line is a suitable transfection host.
Images
Cell Micrograph of ATCC TIB-196, U266B1
Clinical Data
male
Genes Expressed
immunoglobulin; monoclonal antibody; interleukin 6 (interleukin-6, IL-6)
Cellular Products
immunoglobulin; monoclonal antibody; interleukin 6 (interleukin-6, IL-6)
Comments
U266 cells have been reported to produce human IL-6.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.
Medium Renewal: Every 2 to 3 days
Cryopreservation
culture medium 95%; DMSO, 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 12,13
D13S317: 12
D16S539: 10
D5S818: 11,12
D7S820: 11,12
THO1: 5,7
TPOX: 8
vWA: 17
Isotype
IgE; lambda light chain
Name of Depositor
NK Nilsson
Deposited As
Homo sapiens
References

Nilsson K, et al. Established immunoglobulin producing myeloma (IgE) and lymphoblastoid (IgG) cell lines from an IgE myeloma patient. Clin. Exp. Immunol. 7: 477-489, 1970. PubMed: 4097745

Kawano M, et al. Autocrine generation and requirement of BSF-2/IL-6 for human multiple myelomas. Nature 332: 83-85, 1988. PubMed: 3258060

Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128

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