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UACC-812
UACC-812
規格:
價格:
編號:B165901
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
UACC-812
商品貨號
B165901
Organism
Homo sapiens, human
Tissue
mammary gland; breast
培養基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
ductal carcinoma
Age
43 years
Gender
female
Storage Conditions
liquid nitrogen vapor phase
Karyotype
range = 58 to 64; abnormal banding region in 3p
Derivation
This cell line was established by A. Liebovitz and associates in 1986 from breast tissue removed at mastectomy to remove a ductal carcinoma (stage II, grade IV).
Clinical Data
female
Prior to surgery, the patient had received extensive chemotherapy.
Receptor Expression
estrogen receptor (negative), progesterone receptor (negative)
Comments
Prior to surgery, the patient had received extensive chemotherapy.
The cells exhibit a 15 fold amplification of the HER-2/neu oncogene sequence.
They are negative for estrogen receptors, progesterone receptors and P glycoprotein.
Complete Growth Medium
Leibovitz's L-15 medium with 2 mM L-glutamine supplemented with 20 ng/ml human EGF and 20% fetal bovine serum.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days

Note: The cells grow very slowly, and growth is enhanced by using 20% fetal bovine serum and adding epidermal growth factor (20 ng/mL) to the medium.

Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 100%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 8, 11
D5S818: 11, 12
D7S820: 9
THO1: 9
TPOX: 8, 11
vWA: 16
Population Doubling Time
100 hrs
Name of Depositor
A Liebovitz, J Trent
Deposited As
Homo sapiens
Year of Origin
1986
References

Proc. Am. Assoc. Cancer Res. 29: 24, 1988.

Meltzer P, et al. Establishment of two new cell lines derived from human breast carcinomas with HER-2/neu amplification. Br. J. Cancer 63: 727-735, 1991. PubMed: 1674877

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