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當(dāng)前位置: 首頁 > ATCC代理 > UC10-4F10-11 B165903
UC10-4F10-11
UC10-4F10-11
規(guī)格:
價格:
編號:B165903
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
UC10-4F10-11
商品貨號
B165903
Organism
hamster (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The UC10-4F10-11 hybridoma cell line was produced by fusing Sp2/0-Ag14 myeloma cells with spleen cells from an Armenian hamster that had been immunized with the extracellular portion of of murine CTLA-4 fused to a murine IgG2a.
The antibody can be used to detect and quantitate CTLA-4 expression by flow cytometric analysis, and can be used to immunoprecipitate the antigen from CTLA-4 positive cells.
Under conditions of suboptimal T cell receptor (TCR) stimulation, whole antibody or Fab fragments from 4F10 can augment T cell proliferation in allogeneic mixed lymphocyte reactions.
However, when optimal TCR stimulation is present, 4F10 can suppress T cell proliferation.
Derivation
The UC10-4F10-11 hybridoma cell line was produced by fusing Sp2/0-Ag14 myeloma cells with spleen cells from an Armenian hamster that had been immunized with the extracellular portion of of murine CTLA-4 fused to a murine IgG2a.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse CTLA-4
Cellular Products
immunoglobulin; monoclonal antibody; against mouse CTLA-4
Comments
The UC10-4F10-11 hybridoma cell line was produced by fusing Sp2/0-Ag14 myeloma cells with spleen cells from an Armenian hamster that had been immunized with the extracellular portion of of murine CTLA-4 fused to a murine IgG2a.
The antibody can be used to detect and quantitate CTLA-4 expression by flow cytometric analysis, and can be used to immunoprecipitate the antigen from CTLA-4 positive cells.
Under conditions of suboptimal T cell receptor (TCR) stimulation, whole antibody or Fab fragments from 4F10 can augment T cell proliferation in allogeneic mixed lymphocyte reactions.
However, when optimal TCR stimulation is present, 4F10 can suppress T cell proliferation.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.01 mM nonessential amino acids and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG
Name of Depositor
JA Bluestone
References

Walunas TL, et al. CTLA-4 can function as a negative regulator of T cell activation. Immunity 1: 405-413, 1994. PubMed: 7882171

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