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VM-2
VM-2
規(guī)格:
價格:
編號:B165928
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
VM-2
商品貨號
B165928
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody binds to a cell surface antigen present on and characteristic of human basal cells and malignant squamous cells.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human basal cells (skin)
Cellular Products
immunoglobulin; monoclonal antibody; against human basal cells (skin)
Tumorigenic
Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Animals were immunized with human epidermal cells.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
The antibody binds to a cell surface antigen present on and characteristic of human basal cells and malignant squamous cells.
The antibody does not react with normal human fibroblasts, endothelial or hematopoietic cells.
Complete Growth Medium
Culture medium: Dulbecco's modified Eagle's medium with 4.5 g/L
glucose, 0.45 mM sodium pyruvate and 0.01 M HEPES, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium, 90%; DMSO, 10%
Isotype
IgG1
Name of Depositor
Stanford University
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Morhenn V. Monoclonal antibody specific for human basal cell surface antigen. US Patent 4,886,745 dated Dec 12 1989

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