Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Subcultivation Ratio: 1:2
Medium Renewal: Once a week
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Zeigel RF, Clark HF. Electron microscopic observations on a "C"-type virus in cell cultures derived from a tumor-bearing viper. J. Natl. Cancer Inst. 43: 1097-1102, 1969. PubMed: 4982059
Zeigel RF, Clark HF. Histologic and electron microscopic observations on a tumor-bearing viper: establishment of a "C"-type virus-producing cell line. J. Natl. Cancer Inst. 46: 309-321, 1971. PubMed: 5165585
Clark HF, et al. Comparative characterization of a C-type virus-producing cell line (VSW) and a virus-free cell line (VH2) from Vipera russelli. J. Natl. Cancer Inst. 51: 645-654, 1973. PubMed: 4358139
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
