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WAPS 12.2
WAPS 12.2
規(guī)格:
價(jià)格:
編號(hào):B165934
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
WAPS 12.2
商品貨號(hào)
B165934
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human P-selectin (CD62P)
Cellular Products
immunoglobulin; monoclonal antibody; against human P-selectin (CD62P)
Comments
Animals were immunized with P-selectin transfected mouse L1-2 pre-B cells.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody blocks leukocyte adhesion to P-selectin and to platelets.
It recognizes P-selectin of a variety of mammals.
Complete Growth Medium
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain
1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium
pyruvate supplemented with 0.01 mM non-essential amino acids and 0.05 mM
2-mercaptoethanol, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype
IgG1
Name of Depositor
EC Butcher
Deposited As
mouse (B cell); mouse (myeloma)
References

Jutila MA, et al. Cell surface P- and E-selectin support shear-dependent rolling of bovine gamma/delta T cells. J. Immunol. 153: 3917-3928, 1994. PubMed: 7523509

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