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WERI-Rb-1
WERI-Rb-1
規格:
價格:
編號:B165945
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
WERI-Rb-1
商品貨號
B165945
Organism
Homo sapiens, human
Tissue
eye, retina
培養基
Cell Type
retinoblastoma
Product Format
frozen
Morphology
grape-like clusters of round cells
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
retinoblastoma
Age
1 year
Gender
female
Ethnicity
Caucasian
Applications
This cell line is a suitable transfection host.
Storage Conditions
liquid nitrogen vapor phase
Karyotype
This is a near diploid line. The modal chromosome number is 47 occurring at 38%, and the rate of polyploidy is 9%. Fifteen to sixteen marker chromosomes are present in all cells. They are t(1,?), t(3p,5q), der(3)t(?q29;?), t(3q,?), 5q+, i(6p), t(7q,?), 9q+, t(10q,21q), 16q+ and five to six others. Normal chromosomes 3, 10, 13 and 16 are absent. There are two copies of the X chromosome. No Y chromosomes were detected in QM stained preparations
Derivation
The WERI-Rb-1 line is one of two human retinoblastoma cell lines established in 1974 by R.M. McFall and T.W. Sery.
Clinical Data
1 year
Caucasian
female
Tumorigenic
Yes
Effects
Yes, in rabbits
Comments
The cells survive culturing in Difco Bacto-Agar but do not form colonies.
Scanning electron microscopy reveals some variation in the number and frequency of surface blebs, lamellipodia and microvilli.
The line is of interest in studies of cell differentiation, animal models of tumor therapy and biochemical evaluations.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 - 3 X 105 viable cells/mL. Maintain cell density between 1 X 105 and 1 to 2 X 106 viable cells/mL.
Medium Renewal: Every 3 to 4 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 37°C
Isoenzymes
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 0
Name of Depositor
TW Sery
Deposited As
Homo sapiens
Year of Origin
1974
References

McFall RC, et al. Characterization of a new continuous cell line derived from a human retinoblastoma. Cancer Res. 37: 1003-1010, 1977. PubMed: 844036

McFall RC, et al. Scanning electron microscopic observation of two retinoblastoma cell lines. Cancer Res. 38: 2827-2835, 1978. PubMed: 679190

Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024

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