Comments
Animals were immunized with the G-361 human human melanoma cell line see ATCC CRL-1424).
Spleen cells were fused with P3/NS1/1-Ag4-1 myeloma cells.
The antibody reacts with antigens associated with human melanoma cells.
The antibody precipitates antigens of 108000 and 38000 daltons from G-361 lysates.
It reacts with human melanoma cell lines and with WISH (human amnion cell line with HeLa markers) and Rc2a (human acute myelomonocytic leukemia cell line).
It did not react with other line tested or with surgical specimens of non-melanoma neoplasms or with normal tissue.
Most surgical specimens of melanoma were stained positively with WI-MN-1.
