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XMMCO-791

規格:

價格:

編號:B165970

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

XMMCO-791

商品貨號

B165970

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Cell Type

hybridoma: B lymphocyte

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

sarcoma, Carcinoma

Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Applications

The antibody reacts with a tumor associated antigen present in human colorectal carcinoma.

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against colorectal carcinoma associated tumor antigen

Cellular Products

immunoglobulin; monoclonal antibody; against colorectal carcinoma associated tumor antigen

Comments

Animals were immunized with the 791T osteogenic sarcoma cell line (see ATCC CRL-7798).

Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.

The antibody reacts with a tumor associated antigen present in human colorectal carcinoma.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Cryopreservation

culture medium 95%; DMSO, 5%

Culture Conditions

Temperature: 37.0°C

Isotype

IgG2b

Name of Depositor

Xoma Corporation

Deposited As

mouse (B cell); mouse (myeloma)

U.S. Patent Number

4,708,862

References

Baldwin RW. Radioimmuno detection of human cancers using anti-tumor monoclonal antibody. US Patent 4,708,862 dated Nov 24 1987

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