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XMMEN-OE5 [AAB-647, AAB647]
XMMEN-OE5 [AAB-647, AAB647]
規格:
價格:
編號:B165971
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
XMMEN-OE5 [AAB-647, AAB647]
商品貨號
B165971
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody neutralizes gram negative endotoxin activity, and is protective against gram negative sepsis and therapeutic for gram negative sepsis in mice and humans.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against lipopolysaccharide endotoxin of gram negative bacteria
Cellular Products
immunoglobulin; monoclonal antibody; against lipopolysaccharide endotoxin of gram negative bacteria
Tumorigenic
Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with killed E. coli (0111 strain, J5 mutant) cells.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody neutralizes gram negative endotoxin activity, and is protective against gram negative sepsis and therapeutic for gram negative sepsis in mice and humans.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium, 90%; DMSO, 10%
Isotype
IgM
Name of Depositor
Regents of the University of California
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Young LS. Monoclonal antibodies specific for lipid-A determinants of gram negative bacteria. US Patent 4,918,163 dated Apr 17 1990

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