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XR-V15B
XR-V15B
規格:
價格:
編號:B165975
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
XR-V15B
商品貨號
B165975
Organism
Cricetulus griseus, hamster, Chinese
Cell Type
fibroblast
培養基
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Derivation
XR-V15B is a X-ray sensitive Chinese Hamster Lung fibroblast cell line isolated from an ethylnitrosourea (ENU)-mutagenized cell population of V79 cells on the basis of its hypersensitivity to X-rays.
Comments
XR-V15B is a X-ray sensitive Chinese Hamster Lung fibroblast cell line isolated from an ethylnitrosourea (ENU)-mutagenized cell population of V79 cells on the basis of its hypersensitivity to X-rays.
These cells belong to X-ray complementation group 5 and are mutant in the p86 subunit of the Ku autoantigen.
Ku has been shown to be involved in DNA double-strand break repair and V(D)J recombination. Cells will revert to wild type on treatment with azacytidine.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:8 to 1:12 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse quickly with 0.25% trypsin, 0.03% EDTA solution, add 1-2 ml additional trypsin solution and allow flasks to set at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor
G Chu
References

Rathmell WK, Chu G. Involvement of the Ku autoantigen in the cellular response to DNA double-strand breaks. Proc. Natl. Acad. Sci. USA 91: 7623-7627, 1994. PubMed: 8052631

Errami A, et al. Ku86 defines the genetic defect and restores X-ray resistance and V(D)J recombination to complementation group 5 hamster cell mutants. Mol. Cell. Biol. 16: 1519-1526, 1996. PubMed: 8657125

Smider V, et al. Restoration of X-ray resistance and V(D)J recombination in mutant cells by Ku cDNA. Science 266: 288-291, 1994. PubMed: 7939667

Zdzienicka MZ, et al. Characterization of an X-ray-hypersensitive mutant of V79 Chinese hamster cells. Mutat. Res. 194: 239-249, 1988. PubMed: 2460764

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