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Y3-Ag 1.2.3
Y3-Ag 1.2.3
規(guī)格:
價格:
編號:B165983
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Y3-Ag 1.2.3
商品貨號
B165983
Organism
Rattus norvegicus, rat
Cell Type
B lymphoblast
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
plasmacytoma; myeloma
Strain
Lou
Applications
No commercial use or third party distribution is permitted without the concurrence of C. Milstein.
The cells are resistant to 8-azaguanine, are HAT sensitive and can be used as a fusion partner for rat B cells to make rat - rat hybridomas.
Tested and found negative for ectromelia virus (mousepox).
Derivation
This is a derivative of the S210 myeloma developed by C. Milstein.
Genes Expressed
immunoglobulin
Cellular Products
immunoglobulin
Comments
This is a derivative of the S210 myeloma developed by C. Milstein.
No commercial use or third party distribution is permitted without the concurrence of C. Milstein.
The cells are resistant to 8-azaguanine, are HAT sensitive and can be used as a fusion partner for rat B cells to make rat - rat hybridomas.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
kappa light chain
Name of Depositor
K Yamada
Deposited As
Rattus sp.
References

Galfre G, et al. Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG. Nature 277: 131-133, 1979. PubMed: 310519

Bazin H, et al. Transplantable immunoglobulin-secreting tumours in rats. I. General features of LOU-Ws1 strain rat immunocytomas and their monoclonal proteins. Int. J. Cancer 10: 568-580, 1972. PubMed: 4668496

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