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Y79
Y79
規格:
價格:
編號:B165985
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Y79
商品貨號
B165985
Organism
Homo sapiens, human
Tissue
eye, retina
培養基
Cell Type
retinoblastoma
Product Format
frozen
Morphology
multicellular clusters
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
retinoblastoma
Age
2.5 years
Gender
female
Ethnicity
Caucasian
Karyotype
hypertriploidy, with abnormalities including dicentrics, breaks, pulverizations and minutes
Derivation
The Y79 line was isolated by T.W. Reid and associates in January 1971 by explant culture of a primary tumor from the right eye obtained immediately after enucleation.
Clinical Data
2.5 years
Caucasian
female
The donor had a strong maternal family history of retinoblastoma.
Comments
The donor had a strong maternal family history of retinoblastoma.
Ultrastructural features including nuclear membrane infoldings, triple membrane structures, microtubules, large coated vesicles, centrioles, basal bodies and annulate lamellae were reportedly similar to those of the original tumor.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Allow aggregates to settle to the bottom of the flask. Remove supernatant and discard. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 13,14
D5S818: 11,12
D7S820: 8,9
THO1: 6,9.3
TPOX: 8
vWA: 15,18
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
PGM1, 1
Name of Depositor
B Gallie
Deposited As
Homo sapiens
References

Reid TW, et al. Characteristics of an established cell line of retinoblastoma. J. Natl. Cancer Inst. 53: 347-360, 1974. PubMed: 4135597

Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024

Wong HK, Ziff EB. The human papillomavirus type 16 E7 protein complements adenovirus type 5 E1A amino-terminus-dependent trasactivation of adenovirus type 5 early genes and increases ATF and Oct-1 DNA binding activity. J. Virol. 70: 332-340, 1996. PubMed: 8523545

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