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當(dāng)前位置: 首頁(yè) > ATCC代理 > SU-DHL-6 B167210
SU-DHL-6
SU-DHL-6
規(guī)格:
價(jià)格:
編號(hào):B167210
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
SU-DHL-6
商品貨號(hào)
B167210
Organism
Homo sapiens, human
Tissue
peritoneal effusion; derived from metastatic site: peritoneal cavity
培養(yǎng)基
Cell Type
B lymphocyte
Product Format
frozen
Morphology
lymphoblast-like
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
large cell lymphoma; diffuse mixed histiocytic and lymphocytic lymphoma; follicular B cell lymphoma
Gender
male
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
This is a pseudodiploid cell line with a modal chromosome number of 47, a polyploidy rate of approximately 9% and one copy of the X chromosome. No Y chromosome was observed in any of the analyzed cells. A derivative t(14;18)(q32;q21) chromosome was present in most of the examined cells. Other derivative chromosomes were generally consistent with previous studies, these include: del(6)(p23), del(9)(p21.1?), add(11)(q25), i(17q) [or t(6?;17)(p10;q10)?] and del(18)(q21)?. [PubMed: 3881165]
Derivation
The DHL cell lines were successfully established in continuous suspension culture from 10 patients with a histopathological diagnosis of diffuse histiocytic lymphoma (DHL).
Clinical Data

43 years
Caucasian
male

Genes Expressed
monoclonal cytoplasmic immunoglobulin: IgM; lambda light chain
Comments

All of the DHL cell lines were negative for the presence of Epstein-Barr virus (EBV) genomes.
SU-DHL-6 possesses a t(14;18)(q32;q21) translocation and demonstrates an unexpected recombination within its heavy chain gene locus that may be the interchromosomal breakpoint.
In SU-DHL-6, the t(14;18) translocation juxtaposes a truncated bcl-2 gene with J6 in a tail-to-head configuration.

The deregulated expression of the altered bcl-2 gene may play a critical role in the disordered growth and differentiation of follicular B cell lymphoma.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing

Cultures can be maintained by the addition of fresh medium. An inoculum of 8 X 104 to 3 x 105 cells/mL is recommended. Subculture when cell concentration is between 7 x 105 and 1 x 106 cells/mL.

Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended.
Medium renewal: Every 3 to 4 days

Cryopreservation
Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% (v/v) DMSO
Storage Temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile

D5S818: 12
D13S317: 12,14
D7S820: 10
D16S539: 11,12
vWA: 14,17
THO1: 6,9.3
TPOX: 11,12
CSF1PO: 10
Amelogenin: X

Population Doubling Time
approximately 29 hours
Name of Depositor
A Epstein
Year of Origin
1977
References

Hua C, et al. Consequences of the t(14;18) chromosomal translocation in follicular lymphoma: deregulated expression of a chimeric and mutated BCL-2 gene. Oncogene Res. 2(3): 263-275, 1988. PubMed: 3285301

Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220

Kaiser-McCaw Hecht B, et al. Histiocytic lymphoma cell lines: immunologic and cytogenetic studies. Cancer Genet. Cytogenet. 14 (3-4): 205-218, 1985. PubMed: 3881165

Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039

Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794

Bakhshi A, et al. Cloning the chromosomal breakpoint of t(14;18) human lymphomas: clustering around JH on chromosome 14 and near a transcriptional unit on 18. Cell. 41(3):899-906, 1985. PubMed: 3924412

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