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pAL-14

規格:

價格:

編號:B172199

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pAL-14

商品貨號

B172199

Designations

pAL-14

Species

Rattus norvegicus, rat

培養基

Depositors

M Barbacid

Applications

Two are copies of a HpaII/SacI fragment derived from 5' flanking sequences and used as a probe to detect the diagnostic MnlI RFLP. The HpaII/HpaII fragment is of unknown origin.

This clone has been used to detect the MnlI RFLP indicative of a G to A mutation in codon 12 of the rat Ha-ras-1 locus induced by N-nitroso-N-methylurea.

Vector

Construct size (kb): 3.099999904632568

Insert

DNA: genomic

Insert lengths(kb): 0.3600000143051148

Tissue: mammary tumor

Gene product: Harvey rat sarcoma viral (v-Ha-ras) oncogene homolog(Harvey rat sarcoma virus oncogene) [HRAS]

Insert Size (kb)

0.360

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Contains three 120 bp fragments. Two are copies of a HpaII/SacI fragment derived from 5' flanking sequences and used as a probe to detect the diagnostic MnlI RFLP. The HpaII/HpaII fragment is of unknown origin.

This clone has been used to detect the MnlI RFLP indicative of a G to A mutation in codon 12 of the rat Ha-ras-1 locus induced by N-nitroso-N-methylurea. The normal allele is 206 bp and the mutant is 280 bp.

References

Zarbl H, et al. Direct mutagenesis of Ha-ras-1 oncogenes by N-nitroso-N-methylurea during initiation of mammary carcinogenesis in rats. Nature 315: 382-385, 1985. PubMed: 3923365

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