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pGbetaS1.3 [pGBS1.3]
pGbetaS1.3 [pGBS1.3]
規格:
價格:詢價
編號:B173198
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pGbetaS1.3 [pGBS1.3]
商品貨號
B173198
Designations
pGbetaS1.3 [pGBS1.3]
GenBank Number

M18054

培養基
Species
Homo sapiens, human
Applications
A 284 bp SstI fragment and a 630 bp HinfI/BglI fragment were used to detect polymorphisms with PstI and PvuII, respectively.
Vector
Construct size (kb): 3.599999904632568
Insert
DNA: cDNA
Insert lengths(kb): 0.7400000095367432
Tissue: reticulocyte
Gene product: spectrin, beta, erythrocytic (includes sperocytosis, clinical type I) [SPTB]
Alleles: A1, A2, B1, B2
Insert Size (kb)
0.740
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Insert contains the following sites separated by (bp) (approx): (SmaI)- 50- HinfI- 10- HinfI- 100- BglI- 80- SstI- 240- PstI- 50- SstI- 290- HinfI- 20- PstI- 15- (SmaI).
Restriction digests of the clone give the following sizes (kb): PstI--3.2, 0.43; HindIII--3.7; EcoRI/HindIII--2.8, 0.84; EcoRI/BamHI--2.85, 0.82; EcoRI--3.7.
Insert contains human beta-spectrin cDNA corresponding to an internal region of the protein from beta-9 through beta-12.
A 284 bp SstI fragment and a 630 bp HinfI/BglI fragment were used to detect polymorphisms with PstI and PvuII, respectively.
Detected a 7.8 kb message in RNA blots from two leukemia cell lines (K562 and HEL).
Enzyme(s) not detecting polymorphism: several including BamHI and EcoRI.
References

Prchal JT, et al. Isolation and characterization of cDNA clones for human erythrocyte beta-spectrin. Proc. Natl. Acad. Sci. USA 84: 7468-7472, 1987. PubMed: 3478706

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