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當(dāng)前位置: 首頁 > ATCC代理 > pCarMV-1C B173348
pCarMV-1C
pCarMV-1C
規(guī)格:
價格:詢價
編號:B173348
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pCarMV-1C
商品貨號
B173348
Designations
pCarMV-1C
Species
Carnation mottle virus
培養(yǎng)基
Depositors
TJ Morris
Vector
Construct size (kb): 6.550000190734863
Insert
DNA: cDNA
Insert lengths(kb): 3.849999904632568
Gene product: genome, 90%+ complete
Insert Size (kb)
3.850
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Insert contains the following sites separated by (bp)(approx): EcoRI-145- EcoRV-130- NcoI-430- PvuII-315- BglII-70- XhoI-1600- EcoRI-50- KpnI-50- HindIII-200- HincII-840- PstI. Enzymes not cutting insert include BamHI, SmaI, SalI, PstI and ClaI.
Restriction digests of the clone give the following sizes (kb): BamHI--6.5; PstI--3.9, 2.7; EcoRI--3.9, 2.7.
Isolated from Chenopodium quinoa. This is not a full-length clone and is not infectious.
pUC8 dT-tailed at PstI; cut with BamHI; 1st strand synthesis; dC-tailed; cut with EcoRI. pBR322 dG-tailed at PstI; cut with EcoRI; 752 bp fragment = 2nd strand primer. This was hybridized to dC-tailed cDNA, ligated at EcoRI; 2nd strand synthesis.
Classification
Carmovirus
References

Carrington JC, Morris TJ. Complementary DNA cloning and analysis of carnation mottle virus RNA. Virology 139: 22-31, 1984.

Guilley H, et al. Nucleotide sequence and genome organization of carnation mottle virus RNA. Nucleic Acids Res. 13: 6663-6677, 1985. PubMed: 3840587

T J Morris, personal communication

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