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pEGsph50-2 [NEB808]

規格:

價格:詢價

編號:B173684

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

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甘油

平板

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產品名稱

pEGsph50-2 [NEB808]

商品貨號

B173684

Designations

pEGsph50-2 [NEB808]

Species

Neisseria lactamica Hollis et al.

培養基

Depositors

New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc.

Applications

produces protein modification methylase NlaIII

produces protein restriction endonuclease SphI

Vector

Construct size (kb): 9.699999809265137

Insert

DNA: genomic

Insert lengths(kb): 1.0

Gene product: modification methylase NlaIII [sphIR]

Target Gene: modification methylase NlaIII, restriction endonuclease SphI

Insert Size (kb)

1.0

Media

ATCC Medium 1065 (see below) plus ampicillin (50.0 mcg/ml) plus kanamycin (20.0 mcg/ml) ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of p(SYX20)NlaIIIM+ give the following sizes (kb): BamHI--8.8; BamHI/SalI--8.0, 1.10; HindII--4.6, 4.2; BspHI/HindIII--4.6, 4.2.

Restriction digests of pEGsph50-2 give the following sizes (kb): BamHI--uncut; BamHI/SalI--8.0; HindIII--7.6; BspHI/HindIII--7.4.

Expression of the NlaIII methylase protects the host DNA from digestion with by the SphI nuclease. The methylase is expressed constituitively from the tetR promoter.

References

Guthrie EP. Isolated DNA encoding the SphI restriction endonuclease and related methods for producing the same. US Patent 5,262,318 dated Nov 16 1993

Xu SY, Nwankwo DO. Method for cloning and producing the AatII and AluI restriction endonuclease and methylase and related method for overexpressing restriction endonuclases. US Patent 5,405,768 dated Apr 11 1995

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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