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pGSRK-1
pGSRK-1
規格:
價格:詢價
編號:B173808
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pGSRK-1
商品貨號
B173808
Designations
pGSRK-1
Species
Rattus norvegicus, rat
培養基
Depositors
RE Miller
Applications
This clone has been used to isolate mouse genomic glutamine synthetase clones.
Vector
Construct size (kb): 6.0
Name of clone: pGSRK-1
Gene symbol: GLUL
Cloned from: rat kidney
Name of vector: pBR322
Insert site(s): PstI
Insert ends: GC tails
Source of insert DNA: cDNA
Markers: tetR
Insert
DNA: cDNA
Insert lengths(kb): 1.60
Tissue: kidney
Gene product: glutamate-ammonia ligase (glutamine synthase)(glutamine synthetase, glutamate-ammonia ligase, glutamine synthase) [GLUL]
Insert Size (kb)
1.60
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed in Escherichia coli HB101 containing plasmid
Comments
The PstI site distal to the vector EcoRI site was lost during cloning. The insert contains BamHI (2), EcoRI, EcoRV, and PvuII sites.
-Biochem. Biophys. Res. Commun. 134: 146-151, 1986

Restriction digests of the clone give the following sizes (kb): PstI--6.0; BamHI--3.8, 2.25; EcoRI--4.4, 2.1; HindIII--6.0; EcoRV--4.2, 2.5.
-ATCC staff

This clone has been used to isolate mouse genomic glutamine synthetase clones. It detects mRNAs of about 3.2 kb and 1.6 kb inHTML mouse 3T3-L1 cells and rat liver cells.
-Mol. Cell. Endocrinol. 47: 49-57, 1986
References

Bhandari B, et al. Cloning, nucleotide sequence, and potential regulatory elements of the glutamine synthetase gene from murine 3T3-L1 adipocytes. Proc. Natl. Acad. Sci. USA 85: 5789-5793, 1988. PubMed: 2901089

Burns DM, et al. Selection of a rat glutamine synthetase cDNA clone. Biochem. Biophys. Res. Commun. 134: 146-151, 1986. PubMed: 2418824

Bhandari B, et al. Glutamine synthetase mRNA in cultured 3T3-L1 adipocytes: complexity, content and hormonal regulation. Mol. Cell. Endocrinol. 47: 49-57, 1986. PubMed: 2875005

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