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pT7/Nco

規(guī)格:

價格:詢價

編號:B174053

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pT7/Nco

商品貨號

B174053

Designations

pT7/Nco

Depositors

RS Haun

培養(yǎng)基

Other IDs

Nucleotide (GenBank) : U25269 Expression cloning vector pT7/Nde, complete sequence.

Biosafety Level

Vector Information

Size (kb): 2.9000000953674320

Vector: pT7/Nco (plasmid)

Promoters: Promoter SP6

Construction: pGEM-3, pET-3d

Marker(s):ampR

Construct size (kb): 2.900000095367432

Features: marker(s): ampR
promoter: SP6
promoter: T7 (phi10)
replicon: pMB1
MCS: HindIII...XbaI
ribosome-binding site: gene 10

Applications

expression vector

Comments

Restriction digests of the clone give the following sizes (kb): HindIII--2.9; BamHI--2.9; NcoI--2.9.

The NcoI site (CCATGG) includes the initiator methionine codon. The NcoI cloning site can also be used in conjunction with the downstream BamHI site.

Differs from pT7/NdeI (ATCC 87050) only in the cloning site and reading frame for the insert.

Expression vector, maintained at high copy number.

The order of the major features of the plasmid is: SP6 - gene 10 RBS - T7 - NcoI - BamHI - pMB1 ori - ampR.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Hong JX, et al. Effect of ADP-ribosylation factor amino-terminal deletions on its GTP-dependent stimulation of cholera toxin activity. J. Biol. Chem. 269: 9743-9745, 1994. PubMed: 8144566

Shipped

freeze-dried

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