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Influenza A virus (H1N1)
Influenza A virus (H1N1)
規格:
價格:詢價
編號:B174064
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Influenza A virus (H1N1)
商品貨號
B174064
Classification
Orthomyxoviridae, Influenzavirus A
Agent
Influenza A virus (H1N1)
培養基
Strain
A/Swine/1976/31 (TC-adapted)
Applications
Emerging infectious disease research
Respiratory research
Influenza Research
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Derived by adaptation of egg-passage A/Swine/1976/31 (ATCC VR-99) to MDCK (ATCC CCL-34) cells. (Note: ATCC VR-99 and ATCC VR-1682 have not been compared with respect to sequence or infectivity in chick embryo and tissue culture).
Product Format
frozen
Storage Conditions
-70°C or colder
Comments
Derived by adaptation of egg-passage A/Swine/1976/31 (ATCC VR-99) to MDCK (ATCC CCL-34) cells. (Note: ATCC VR-99 and ATCC VR-1682 have not been compared with respect to sequence or infectivity in chick embryo and tissue culture).
Effect on Host
CPE (cell rounding, degeneration, sloughing)
CPE (cell rounding, degeneration, sloughing)
Growth Medium
ATCC® Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Recommended Host
Production Host: MDCK (ATCC CCL-34)
Alternate Hosts: Chicken embryo; MDCK and monkey kidney cells (MkK)

Recommended Host:? MDCK (ATCC? CCL-34?)

Growth Conditions
For best results, plate cells 24-48 hours in advance. Infect at 80%-90% confluent (not 100% confluent) using a multiplicity of infection (MOI) of 0.01 to 1.0. Incubate at 35.0°C in a humidified, 5% CO2 atmosphere for 2-4 days, until CPE are well advanced in 100% of the culture. In TC, ATCC VR-1682 should be grown in serum free media that contains 1 ug/ml TPCK-treated trypsin, 0.125% BSA and 1% HEPES (1 molar stock).
Name of Depositor
M.T. Coleman and ATCC
Source
Hog in Iowa, 1931.
Year of Origin
1976
References

Jensen KE, Peterson WD Jr.. Comparative measurements of antigenic differences among human and swine influenza viruses. J. Immunol. 78: 365-372, 1957. PubMed: 13449325

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