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pERT87-30
pERT87-30
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B174414
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pERT87-30
商品貨號(hào)
B174414
Designations
pERT87-30
Species
Homo sapiens, human
培養(yǎng)基
Depositors
LM Kunkel
Vector
Construct size (kb): 4.599999904632568
Insert
DNA: genomic
Insert lengths(kb): 1.799999952316284
Gene product: DNA Segment, single copy (and related walk clones (within DMD gene)) [DXS164]
Alleles: N1, N2, A1, A2, B1, D1, D2, E2, G1, J1, J2, K1, O1, O2, E1, G2, K2, B2
Insert Size (kb)
1.800
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: frozen
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--2.7, 1.8; EcoRI--4.6; BamHI--4.6; PstI--4.6; SmaI--4.6; consistent with deposited DNA.
This construct is not stable when freeze-dried and is distributed from liquid nitrogen stock.
This clone is from within the DMD locus.
This clone is 54.5 kb more pter than pERT87-15.
The polymorphisms detected by this probe are contained within the DMD locus.
The DNA must be extensively nicked to get efficient transfer of the large fragment.
References

Koenig M, et al. Complete cloning of the Duchenne muscular dystrophy (DMD) cDNA and preliminary genomic organization of the DMD gene in normal and affected individuals. Cell 50: 509-517, 1987. PubMed: 3607877

Monaco AP, et al. Localization and cloning of Xp21 deletion breakpoints involved in muscular dystrophy. Hum. Genet. 75: 221-227, 1987. PubMed: 2881877

Louis M Kunkel, personal communication

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