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pBT-1

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價格:詢價

編號:B174589

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

斜面

甘油

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產品名稱

pBT-1

商品貨號

B174589

Designations

pBT-1

Species

human immunodeficiency virus - 1

培養基

Depositors

SM Watanabe

Vector

Construct size (kb): 11.77999973297119

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC18
Intact vector size: 2.686
Type of vector: plasmid
Vector end: SstI
Vector end: SstI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: HindIII...EcoRI, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: L08752

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: human immunodeficiency virus - 1
Gene name: genome, full-length
Contains complete coding sequence?: U
Strain: BRU
Type of DNA: cDNA
Insert end: SstI
Insert end: SstI
Insert size (kb): 9.09
Cross references:

Insert lengths(kb): 9.09000015258789

Insert Size (kb)

9.090

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: frozen

Comments

Restriction digests of the clone give the following sizes (kb): SstI--9.0, 2.7; EcoRI--6.2, 4.0, 1.0; HindIII--6.2, 3.1, 1.4, 0.64.

Contains the proviral genome from bp 33 to 9129 (numbered according to Wain-Hobson et al., 1985 (Cell 40:9-17). The insert is oriented with the 5' end closer to the EcoRI site in the polylinker of the vector.

Cloned from human immunodeficiency virus 1BRU. Contains the proviral genome from bp 33 to 9129.

This is a subclone of lambdaJ19.

References

Watancke SM. Expression and diagnostic use of pENV-3 encoded peptides which are immunologically reactive with antibodies to LAV. US Patent 4,784,941 dated Nov 15 1988

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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