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YEp437 [pRB698]

規(guī)格:

價格:

編號:B175181

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

YEp437 [pRB698]

商品貨號

B175181

Designations

YEp437 [pRB698]

Depositors

D Botstein

培養(yǎng)基

Biosafety Level

Vector Information

Size (kb): 13.9000005722045900

Vector: YEp437 (plasmid)

Construction: pSI4, YEp6

Marker(s):ampR,HIS3,LEU2-d

Construct size (kb): 13.90000057220459

Features: marker(s): ampR, LEU2-d, HIS3
replicon: pMB1, 2 micron

Applications

YE-type (episomal) shuttle vector

shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--12.0, 1.8; EcoRI--6.2, 4.4, 2.4, 0.8; SalI--14.0.

LEU2-d is a promoter-defective allele of the yeast LEU2 gene, which increases plasmid copy number to 200-300 copies in the presence of LEU2 selection (host grown in medium lacking leucine).

YE-type shuttle vector.

The order of the major features in this plasmid is: LEU2-d - ori(2 micron) - HIS3 - SalI site - ampR.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Ma H, et al. Plasmid construction by homologous recombination in yeast. Gene 58: 201-216, 1987. PubMed: 2828185

Rose AB, Broach JR. Propagation and expression of cloned genes in yeast: 2-micron circle-based vectors. Methods Enzymol. 185: 234-279, 1990. PubMed: 2199781

Shipped

freeze-dried

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