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SMH-5

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價格:詢價

編號:B175182

品牌:Mingzhoubio

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DNA

RNA

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產品名稱

SMH-5

商品貨號

B175182

Designations

SMH-5

GenBank Number

X83618

培養基

Species

Homo sapiens, human

Depositors

FG Hegardt

Vector

Construct size (kb): 5.019999980926514

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites:
Polylinker sites:
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: HMGCS2
Gene name: 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2
(mitochondrial)
Contains complete coding sequence?: Y
Chromosome: 1; Localization: 1 p13-p12
Tissue: liver
Type of DNA: cDNA
Insert end: Modification: EcoRI
Insert end: Modification: EcoRI
Insert size (kb): 2.058
Cross references: DNA Seq. Acc.: X83618
Nucleotides 1-2058 of the insert correspond to
nucleotides 1-2058 of X83618.

Insert lengths(kb): 2.058000087738037

Tissue: liver

Gene product: 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial) [HMGCS2]

Insert Size (kb)

2.058

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.1; HindIII--3.2, 0.9, 0.9; KpnI--3.2, 1.8; PstI--4.4, 0.55; StuI--5.0.

The insert contains the following restriciton sites (approximate kb from the 5' end): HindIII--0.82,1.60; StuI--1.12; KpnI--1.52; PstI--1.59.

Use of BLAST shows that 99% (2468/1476) of nt 485-1960 in X83618 are identical to nt 1-1474 of U12789.

References

Mascaro C, et al. Molecular cloning and tissue expression of human mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase. Arch. Biochem. Biophys. 317: 385-390, 1995. PubMed: 7893153

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