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RATREN
RATREN
規(guī)格:
價格:詢價
編號:B175642
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
RATREN
商品貨號
B175642
Designations
RATREN
GenBank Number

J02941

培養(yǎng)基
Species
Rattus norvegicus, rat
Depositors
KR Lynch
Vector
Construct size (kb): 4.400000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-4Z
Intact vector size: 3.000
Type of vector: plasmid
Vector end: BamHI
Vector end: HindIII
Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI
SphI HindIII
Polylinker sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI
SphI HindIII
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: rat
Gene symbol: REN
Genomic copy number: unique
Gene name: renin
Contains complete coding sequence?: Y
Strain: Sprague-Dawley
Tissue: kidney, male
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 1.4
Cross references: DNA Seq. Acc.: J02941
Nucleotides 1-1432 of the insert correspond to
nucleotides 184-1615 of J02941.
Insert lengths(kb): 1.399999976158142
Tissue: kidney, male
Gene product: renin [REN]
Alleles: A1, A2
Insert Size (kb)
1.400
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--3.0, 1.4; PstI--3.4, 0.95; BamHI--4.3.
Constructed by isolating a 1.4 kb EcoRI fragment from a partial digest of a clone in lambdagt10 and cloning the fragment into a pBluescript vector. A 1.4 kb BamHI/HindIII fragment was excised and cloned into pGEM-4Z.
References

Burnham CE, et al. Molecular cloning of rat renin cDNA and its gene. Proc. Natl. Acad. Sci. USA 84: 5605-5609, 1987. PubMed: 3039496

Kevin R Lynch, personal communication

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