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pMWNT3 plasmid in E. coli GC10

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編號:B175730

品牌:Mingzhoubio

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產品名稱

pMWNT3 plasmid in E. coli GC10

商品貨號

B175730

Designations

pMWNT3 plasmid in E. coli GC10

GenBank Definition

Mutated version- (tryptophan 539 to leucine)

培養基

GenBank Number

M32502

Species

Mus musculus, mouse

Depositors

Roel Nusse

Vector

Construct size (kb): 4.6

Insert

DNA: cDNA

Gene: Wingless-type MMTV integration site family member 3, Wnt-3

Source: mouse

Insert size (kb): 1.4

Excise insert: EcoRI

Putative ID

wingless-related MMTV integration site 3

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

?Frozen glycerol stock of Escherichia coli GC10 containing the plasmid.

Comments

This is a clone of the mouse Wnt-3 cloned into the EcoRI site of the pGEM-3Zf vector.

The insert corresponds to approximately nt 170-1570 of the Wnt-3 sequence in Genbank (M32502).

Restriction digests of this clone give the following sizes (in kb): SmaI - 4.4, 0.45; EcoRI - 3.5, 1.5. - ATCC staff

Classification

Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod

References

Roelink H, et al. Molecular cloning and chromosomal localization to 17q21 of the human WNT3 gene. Genomics 17: 790-792, 1993.

B189727：pMWNT3A plasmid in E. coli GC10

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