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70-38
70-38
規(guī)格:
價格:詢價
編號:B175818
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
70-38
商品貨號
B175818
Designations
70-38
Species
Mus musculus, mouse
培養(yǎng)基
Depositors
CM Disteche
Applications
Isolated from a flow-sorted library enriched for the derivative X(7) chromosome produced by the Cattanach translocation T(X;7)1Ct.
Vector
Construct size (kb): 14.60000038146973
Insert
DNA: genomic
Insert lengths(kb): 9.0
Gene product: DNA segment, Chr X, University of Washington-70 [DXWas70]
Insert Size (kb)
9.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--9.0, 5.8; BamHI--6.4, 5.6, 2.25, 0.7; PstI--14.8; BglI--9.4, 2.4, 1.1 (doublet), 0.8; HindIII--11.0, 2.0, 1.4, 0.4.
In the strains that demonstrate amplification, variant bands are detected in Southern blots when genomic DNA is digested with MspI, HindIII, BglII, TaqI, BglI, BclI, MboI and EcoRI.
This sequence exists in about 20 copies in strains A/J, AKR/J, BALB/cJ, CBA/J, C3H/HeJ, DBA/2J, SWR/J, and C57BL/6J (all on the X chromosome).
In other strains, the number of copies is 80 - 100, with some of the additional sequences being on chromsome 11 (C57BL/10ScSn mdx, C57BL/10J), chromosome 17 (SJL/J, PO) or chromosome 13 (NZB/B1NJ).
Hybridizes to the X chromosome of Mus caroli.
Also detects multiple bands in the human genome.
Isolated from a flow-sorted library enriched for the derivative X(7) chromosome produced by the Cattanach translocation T(X;7)1Ct.
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Disteche CM, et al. Comparison of the physical and recombination maps of the mouse X chromosome. Genomics 5: 177-184, 1989. PubMed: 2571569

Disteche CM, et al. Isolation of mouse X-chromosome specific DNA from an X-enriched lambda phage library derived from flow sorted chromosomes. Cytometry 2: 282-286, 1982. PubMed: 6210515

Disteche CM, et al. Isolation and characterization of two repetitive DNA fragments located near the centromere of the mouse X chromosome. Cytogenet. Cell Genet. 39: 262-268, 1985. PubMed: 2932308

Disteche CM, et al. Translocation and amplification of an X-choromosome DNA repeat in inbred strains of mice. Nucleic Acids Res. 15: 4393-4401, 1987. PubMed: 3588301

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