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pHGN3

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編號:B176250

品牌:Mingzhoubio

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RNA

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產品名稱

pHGN3

商品貨號

B176250

Designations

pHGN3

GenBank Number

M14783

培養(yǎng)基

Species

Vaccinia virus

Depositors

University of Florida, Gainesville, RW Moyer, University of Florida, Gainesville

Applications

contains sequence hemagglutinin

integrating vector

shuttle vector

vector permitting positive selection for integration

vector with sequences for protein export

Vector

Name of vector: pUC9
Intact vector size: 2.700
Type of vector: plasmid
Vector end: HaeII
Vector end: HaeII
Cloning sites: HindIII PstI SalI AccI HincII BamHI SmaI XmaI EcoRI HaeIII
Polylinker sites: HindIII PstI SalI AccI HincII BamHI SmaI XmaI EcoRI HaeIII
Construction: pUC71K
Host range: Escherichia coli

Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1

Cross references: DNA Seq. Acc.: L09128

Insert

Genome: Vaccinia virus
Gene name: hemagglutinin
Contains complete coding sequence?: U
Strain: IHD-J (ATCC VR-156)
Type of DNA: genomic
Insert end: Modification: KpnI linkers
Insert end: Modification: KpnI linkers
Insert size (kb): 1.85

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--4.2; EcoRI--4.2; SalI/HindIII--3.2, 1.0.

Recombinants can be identified by inactivation of the hemagglutinin gene (HA-).

Shuttle vector component of a virulence tester system to be used with the avirulent rabbit poxvirus deletion mutant RPmuhr23HA+ (ATCC VR-2379).

DNA cloned into the MCS is integrated into VR-2379 by homologous recombination, possibly altering the host range and virulence of the virus.

The hemagglutinin insert is a 1.8 kb 5'SalI/3'HindIII fragment containing approximately 443 bp 5' flanking sequence, 988 bp coding sequence interrupted by a polylinker, and 408 bp 3' flanking sequence.

The gene is interrupted at the AccI site (nt 43 of the coding region) by a 47 bp MCS from pUC9.

Constructed by deleting lacZ and the polylinker from pUC9 by a HaeII digest.

The order of the major features in the plasmid is: pUC9 delta lacZ - KpnI - 5' end of insert - PstI/MCS/EcoRI - 3' end of insert - KpnI.

Classification

Poxviridae, Orthopoxvirus

References

Moyer RW, Bloom DC. Biological system for constructing and testing viral vaccines. US Patent 5,212,057 dated May 18 1993

Bloom DC, et al. Identification and characterization of two nonessential regions of the rabbit pox virus genome involved in virulence. J. Virol. 65: 1530-1542, 1991. PubMed: 1847470

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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