Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
These cells are generated from mouse embryonic fibroblasts, STO cell line (ATCC CRL-1503), by a treatment of Mitomycin C.
These cells are provided to be used as feeder cells to support the growth of other cells. They have been treated with Mitomycin C and will not replicate. The cells will begin to deteriorate in 2 to 3 weeks after plating. Once the feeder cells have attached, the culture medium can be changed to accommodate the cells to be supported. Such populations are employed for maintenance of embryonal stem cells such as ES-D3 (ATCC CRL-1934) or teratocarcinoma stem cells (see ATCC CRL-1535 and ATCC CRL-1566) in the undifferentiated state.
It is recommended that the feeder cells be plated 24 hours before use at 6 x 106/T-75 or 2 x 106/T-25 in order to obtain a 100% confluent monolayer for stem cells growth.
Martin GR, Evans MJ. Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro. Proc. Natl. Acad. Sci. USA 72: 1441-1445, 1975. PubMed: 1055416
Teratomas and Differentiation, eds. Michael I. Sherman and David Solter, 13–14. New York: Academic Press, 1975.
Martin GR, et al. The development of cystic embryoid bodies in vitro from clonal teratocarcinoma stem cells. Dev. Biol. 61: 230-244, 1977. PubMed: 590624
