最新中文字幕-精品国产99-国产精品福利在线观看-粉嫩av一区二区三区-日韩精品久久久久-美丽的姑娘国语视频-亚洲欧洲色-国产不卡毛片-三级黄毛片-免费福利在线-日韩av三区-欧美成年人网站-欧洲美女与动交ccoo-成人涩涩软件-秋霞影院午夜老牛影院-婷婷久久伊人-呦呦视频在线观看-www.天天综合-日韩精品高清视频-国产欧美综合在线-欧美日韩一区二区三区在线视频-a猛片免费播放-91九色蝌蚪国产-国产欧美日韩专区-嫩模被强到高潮呻吟不断

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > pPB101 B176508
pPB101
pPB101
規格:
價格:詢價
編號:B176508
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pPB101
商品貨號
B176508
Designations
pPB101
Depositors
HM Temin
培養基
Biosafety Level
2
Vector Information
Size (kb): 12.2799997329711900
Vector: pPB101 (plasmid)
Promoters: Promoter SNV LTR
Construction: pSW210, p60B Sal
Marker(s):ampR,tetS
Construct size (kb): 12.30000019073486
Features: marker(s): ampR
promoter: SNV LTR
replicon: pMB1, SNV
Applications
contains sequence
expression vector
shuttle vector
Comments
The insert contains an internal SalI site about 860 bp from the EcoRI end.
Full length molecular clone of spleen necrosis virus genome in pBR322.
Full-length molecular clone of spleen necrosis virus genome in pBR322.
A plasmid expression vector constructed from spleen necrosis virus, an avian retrovirus.
An EcoRI/BglII double digest yields a 2.0 kb fragment containing an LTR and initiator ATG for the gag protein.
This was constructed by cloning the 7.7 kb SalI fragment from p60B Sal (containing the SalI fragment of clone 60B) into the SalI site of pSW210 (pBR322 containing the AvrII/SalI fragment of pSNV-60B), thus generating an insert with 2 LTRs.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Watanabe S, Temin HM. Construction of a helper cell line for avian reticuloendotheliosis virus cloning vectors. Mol. Cell. Biol. 3: 2241-2249, 1983. PubMed: 6318091

Bandyopadhyay PK, Temin HM. Expression from an internal AUG codon of herpes simplex thymidine kinase gene inserted in a retrovirus vector. Mol. Cell. Biol. 4: 743-748, 1984. PubMed: 6325894

Chen IS, Temin HM. Substitution of 5' helper virus sequences into non-rel portion of reticuloendotheliosis virus strain T suppresses transformation of chicken spleen cells. Cell 31: 111-120, 1982. PubMed: 6297744

O'Rear JJ, et al. Infectious and noninfectious recombinant clones of the provirus of SNV differ in cellular DNA and are apparently the same in viral DNA. Cell 20: 423-430, 1980. PubMed: 6248244

Shipped
freeze-dried
Shipping Information
Distributed: freeze-dried
  • 王經理
    手機號:17280875617
    QQ:1438578920
  • 胡經理
    手機號:13345964880
    QQ:2438244627
  • 周經理
    手機號:17757487661
    QQ:1296385441
  • 于經理
    手機號:18067160830
    QQ:2088210172
  • 沈經理
    手機號:19548299266
    QQ:2662369050
  • 李經理
    手機號:13626845108
    QQ:972239479
  • 曹經理
    手機號:18662422060
    QQ:3671869992