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JVM-13
JVM-13
規格:
價格:詢價
編號:B176639
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
JVM-13
商品貨號
B176639
Organism
Homo sapiens, human
Tissue
peripheral blood
培養基
Cell Type
lymphoblast; immortalized with Epstein-Barr virus (EBV); Epstein-Barr virus (EBV) transformed
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
2 [Cells infected with Epstein-Barr virus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
B-prolymphocytic leukemia (B-PLL)
Age
late 60's
Gender
male
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
Modal chromosome number: 47This is a pseudodiploid human cell line of male origin with a modal chromosome number of 47. Most of the cells contain the derivative chromosomes: add(2q), t(8;13)(p12;q13) and t(8;?)(p12;?). Overall, the karyology was consistent with other published data [PubMed: 3262465].
Images
Derivation
Leukemic cells from a patient with B-prolymphocytic leukemia were immortalized in vitro with Epstein-Barr virus (EBV) in the presence of phorbol-ester, TPA.
Clinical Data
Late 60's
Caucasian
male
Antigen Expression
CD3-, CD5-, CD10-, CD19+, CD20+, CD23+, FMC7+ (verified at ATCC)
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing

Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 104 to 4 x 104 viable cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).


Cryopreservation
70% growth medium; 20% FBS; 10% DMSO. Cell culture tested DMSO is available as ATCC? Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 9,11
D16S539: 11
D5S818: 11
D7S820: 9
THO1: 6,7
TPOX: 10,11
vWA: 16,18
Population Doubling Time
about 58 hours
Name of Depositor
J.V.Melo
References

Melo JV, et al. Two new cell lines from B-prolymphocytic leukaemia: characterization by morphology, immunological markers, karyotype and Ig gene rearrangement. Int. J. Cancer 38: 531-538, 1986. PubMed: 3093393

Melo JV, et al. The establishment of cell lines from chronic B cell leukaemias: evidence of leukaemic origen by karyotypic abnormalities and Ig gene rearrangement. Clin. Exp. Immunol. 73: 23-28, 1988. PubMed: 3262465

Tucker, C.A., et al. Four human t(11;14)(q13;q32)-containing cell lines having classic and variant features of Mantle Cell Lymphoma. Leuk Res. 30(4): 449-457, 2006. PubMed: 16183118.

Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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