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pMTD
pMTD
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B176679
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pMTD
商品貨號(hào)
B176679
Designations
pMTD
GenBank Number

U21960

培養(yǎng)基
Species
Mus musculus, mouse
Depositors
VM Morales, RS Blumberg
Vector
Construct size (kb): 4.400000095367432
Insert
DNA: cDNA
Insert lengths(kb): 1.375
Tissue: thymus, female, 6-8 weeks
Gene product: calcium modulating ligand( calcium-signal modulating cyclophilin ligand, calcium-modulating cyclophilin ligand) [Caml]
Insert Size (kb)
1.375
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.4; EcoRI/HincII--3.0, 1.1, 0.3; HincII--4.4; PstI--3.4, 1.0.
Insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.46; HincII--1.10; NcoI--0.81; PstI--0.97; PvuII--0.35, 0.53, 0.76; SacI--0.42; SmaI--0.18.
Bacteria containing this plasmid grow slowly and yield less DNA than other pBluescript clones. Clones should be grown using low levels of ampicillin (50 mcg/ml) and a single colony should be isolated, avoiding those colonies that grow quickly.
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Kim HS, et al. Cloning of the gene encoding the mouse homologue of the human calcium signal-modulating ligand. Gene 163: 323-324, 1995. PubMed: 7590290

Victor M Morales, personal communication

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