Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Temperature: 37°C
Recommendations for Infection: For best results, pre-treat cells with 25 µg/mL DEAE-Dextran (MP Biomedicals 02195133) for 30 minutes at 23°C. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Add virus dilution to host cells and rock or gently vortex every 15-20 minutes for the first 1-2 hours of incubation at 37°C. Re-suspend cells at an appropriate density. Incubate at 37°C in a humidified 5% CO2 atmosphere.
Incubation: 12-21 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the culture.
Tompkins WAF, Tompkins MB. Feline immunodeficiency virus isolate NCSU.sub.1Lb. US Pat. 5,413,927 dated May 9, 1995.
