Restriction digests of the clone give the following sizes (kb): BamHI--6.6; EcoRI--6.6; SacI--6.2; SacI/XbaI--3.6, 1.8, 1.1, 0.3; XbaI--3.5, 1.6, 0.94.
Contains a truncated version of the CYC1 promoter that very weak and no longer inducible because of a deletion of most of the UAS2 sequence.
High copy number shuttle expression vector.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504
