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Adeno-associated virus 1

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貨期:

編號:B176973

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

斜面

甘油

平板

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產品名稱

Adeno-associated virus 1

商品貨號

B176973

Classification

Parvoviridae, Dependovirus, Adeno-associated virus-1

Deposited As

Adeno-associated (satellite) virus type 1

Agent

Adeno-associated virus 1

Strain

none

Applications

Suggest VR-848, Adenovirus Type 7A, as helper virus, which produces CPE on ATCC CRL-1573 cells.

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation

Pool of RhMk TC infected with SV-15 virus

Product Format

frozen

Storage Conditions

-70°C or colder (or colder)

Comments

Depends upon adenovirus for replication. Suggest VR-848, Adenovirus Type 7A, as helper virus, which produces CPE on ATCC CRL-1573 cells. *Helper virus may be inactivated by heating to 56C for 30 min.

Effect on Host

in vitro effects: Adeno-associated virus shows no independent cytopathic effects

Adenovirus CPE, refractile rounding and sloughing; AAV shows no independent CPE.

Yes, in vitro effects: Adenovirus cytopathic effects, refractile rounding and sloughing

Growth Medium

Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%

Recommended Host

Recommended Host:? HEK-293 (ATCC^? CRL-1573^TM)

Growth Conditions

Atmosphere: 5% CO2 in air recommended

Temperature: 37.0°C

Duration: 2-4 days; For best results cells should be 24 to 48 hours old and 70-90% confluent (not 100% confluent).

Cryopreservation

Storage temperature: -70°C

Name of Depositor

WP Parks

Special Collection

ATCC