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asb4/134

規格:

價格:詢價

編號:B176978

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

asb4/134

商品貨號

B176978

Designations

asb4/134

GenBank Number

X15617

培養基

Species

Mus musculus, mouse

Depositors

AJ Ouellette

Applications

This probe has been used to isolate several cryptdin-related cDNAs from an intestinal cDNA library.

Vector

Construct size (kb): 4.679999828338623

Insert

DNA: cDNA

Insert lengths(kb): 0.3149999976158142

Tissue: adult small intestine

Gene product: defensin-related cryptdin peptide [Defcr]

Alleles: b, a, c

Insert Size (kb)

0.315

Media

ATCC^® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

The insert contains the following sites separated by (bp): (PstI)- 46- AvaII- 54- HinfI- 12- BglII- 2- Sau3A, XhoII- 15- Sau3A- 45- PstI- 18- RsaI- 54- RsaI- 19- BanI- 48- (PstI).

Restriction digests of the clone give the following sizes (kb): PstI--4.5, 0.16 (doublet); EcoRI--4.8; HindIII--4.8; BamHI--4.8; AvaI--4.8; PvuI/NdeI--2.9, 1.8.

The cryptdin mRNA (450 - 500 nt in length) is abundant in Paneth cells and can be detected in the testis and the brain.

The gene maps to the proximal region of chromosome 8, within 1.28-21.27 cM of the Xmv-26 locus.

Contains a open reading frame encoding a highly polar protein of 70 aa with all 6 cysteine residues in the COOH-terminal half. There are about 100 untranslated nucleotides 3', including poly(A).

The insert lacks 115 bp of 5'-terminal sequence of the mRNA (which has a total size of 0.45 kb). This probe has been used to isolate several cryptdin-related cDNAs from an intestinal cDNA library.

Classification

Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod

References

Ouellette AJ, et al. Localization of the cryptdin locus on mouse chromosome 8. Genomics 5: 233-239, 1989. PubMed: 2571573

Ouellette AJ, et al. Developmental regulation of cryptdin, a corticostatin/defensin precursor mRNA in mouse small intestinal crypt epithelium. J. Cell Biol. 108: 1687-1695, 1989. PubMed: 2715173

Ouellette AJ, Lualdi JC. A novel mouse gene family coding for cationic, cysteine-rich peptides: regulation in small intestine and cells of myeloid origin. J. Biol. Chem. 265: 9831-9837, 1990. PubMed: 2351676

Andre J Ouellette, personal communication

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