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pHKHK3a
pHKHK3a
規(guī)格:
價(jià)格:詢價(jià)
編號:B177030
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pHKHK3a
商品貨號
B177030
Designations
pHKHK3a
GenBank Number

X78678

培養(yǎng)基
Species
Homo sapiens, human
Applications
The second polyadenylation signal was used.
Vector
Construct size (kb): 6.400000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCDM8
Intact vector size: 4.356
Type of vector: phagemid
Vector end: BstXI
Vector end: BstXI
Cloning sites: HindIII XbaI XhoI BstXI PstI NotI
Polylinker sites: HindIII XbaI XhoI BstXI --stuffer--BstXI XhoI PstI NotI XbaI
Construction: CMV, SV40
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
replicon: pMB1, 1-587
replicon: M13, <-, 588-1182
marker(s): supF, <-, 1183-1384
promoter: CMV, ->, 1516-2170
promoter for in vitro transcription: T7, ->, 2171-2189
MCS: HindIII...XbaI, ->, 2198-2655
terminator: SV40 splice site and polyadenylation, ->, 2656-3352
replicon: polyoma, 3353-4188
replicon: SV40, 4189-4352
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: KHK
Gene name: ketohexokinase (fructokinase)
Contains complete coding sequence?: Y
Tissue: Hep G2 cell line
Disease: hepatocellular carcinoma
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.899
Cross references: DNA Seq. Acc.: X78678
Nucleotides 1-1899 of the insert correspond to
nucleotides 1-1899 of X78678.
Insert lengths(kb): 1.89900004863739
Tissue: Hep G2 cell line hepatocellular carcinoma
Disease: hepatocellular carcinoma
Gene product: ketohexokinase (fructokinase) [KHK]
Insert Size (kb)
1.899
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): XbaI--4.3, 2.1; HindIII--6.4; BamHI--3.9, 2.4; SacI--4.7, 0.87, 0.7; PstI--4.7, 1.1, 0.5.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.41, SacI--0.54, 1.36; PstI--0.46, 0.88.
Derived from the form A variant transcript (alternately spliced first coding exon). The second polyadenylation signal was used.
References

Bonthron DT, et al. Molecular basis of essential fructosuria: molecular cloning and mutational analysis of human ketohexokinase (fructokinase). Hum. Mol. Genet. 3: 1627-1631, 1994. PubMed: 7833921

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