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pUC-h27OH
pUC-h27OH
規格:
價格:詢價
編號:B177065
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pUC-h27OH
商品貨號
B177065
Designations
pUC-h27OH
GenBank Number

M62401

培養基
Species
Homo sapiens, human
Depositors
DW Russell
Applications
contains sequence cytochrome P-450, sterol 27-hydroxylase cytochrome P450, subfamily XXVIIA (steroid 27-hydroxylase, cerebrotendinous xanthomatosis), polypep, sterol 27-hydroxylase
Vector
Construct size (kb): 4.579999923706055
Insert
DNA: cDNA
Insert lengths(kb): 1.884999990463257
Tissue: human prostate
Gene product: cytochrome P-450, sterol 27-hydroxylase( cytochrome P450, subfamily XXVIIA (steroid 27-hydroxylase, cerebrotendinous xanthomatosis), polypep, sterol 27-hydroxylase) [h27OH]
Target Gene: cytochrome P-450, sterol 27-hydroxylase
Insert Size (kb)
1.885
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.9; HindIII--3.0, 1.6; XbaI--4.6; BamHI--3.0, 1.6; PstI--2.9, 0.8, 0.7, 0.2.
DNA sequence analysis of hybridization-positive clones predicted a human sterol 27-hydroxylase consisting of a 33-amino-acid mitochondrial signal sequence followed by a mature protein of 498 amino acids.
References

Cali JJ, Russell DW. Characterization of human sterol 27-hydroxylase. A mitochondrial cttochrome P-450 that catalyzes multiple oxidation reaction in bile acid biosynthesis. J. Biol. Chem. 266: 7774-7778, 1991. PubMed: 1708392

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