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pLKC480

規格:

價格:詢價

編號:B177499

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

加入購物車

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產品名稱

pLKC480

商品貨號

B177499

Designations

pLKC480

Depositors

AA Tiedman, A Shiau

培養基

Biosafety Level

Vector Information

Size (kb): 9.8999996185302730

Vector: pLKC480 (plasmid)

Promoters: Promoter none

Construction: A lacZY plasmid, Tn5

Marker(s):ampR,kanR

Construct size (kb): 9.899999618530273

Features: insert detection: lacZ
marker(s): ampR, kanR
promoter: none
replicon: pMB1
terminator: none
enhancer: none

Applications

contains easily purifiable cassette(s) for construction

integrating vector

vector permitting construction of fusion proteins

vector permitting visual detection of recombinants

Comments

The 6.3 kb lacZY-kanR cassette is released with SmaI.

Restriction digests of the clone give the following sizes (kb): EcoRI--10.05; BamHI--10.05; PstI--6.0, 4.4; HindIII--10.05.

One of a series of lacZY fusion vectors (ATCC 37594-37596) that allow fusions in one of three reading frames and retain flanking DNA for homologous recombination into the Escherichia coli genome.

A 1.3 kb HindIII/NruI kanR fragment (with HindIII site filled in) was inserted at the NruI site of a lacZY fusion plasmid.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Tiedeman AA, Smith JM. lacZY gene fusion cassettes with kanR resistance. Nucleic Acids Res. 16: 3587, 1988. PubMed: 3131742

Shipped

freeze-dried

Shipping Information

Distributed: freeze-dried

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