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pMAP29 [112-12A, 3732-3-7A]

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價格:

編號:B177655

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

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甘油

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產品名稱

pMAP29 [112-12A, 3732-3-7A]

商品貨號

B177655

Designations

pMAP29 [112-12A, 3732-3-7A]

Species

Bacillus thuringiensis subsp. kurstaki de Barjac and Lemille

培養基

Depositors

Monsanto Co., FJ Perlak, Monsanto Co.

Applications

produces protein crystal protein (delta endotoxin)

Vector

Construct size (kb): 35.0

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGS9
Intact vector size: 30.000
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI
Polylinker sites:
Construction: pACYC184
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: P15A
other: IS50L, <-
marker(s): kanR, ->
restriction site: BamHI
other: IS50R, ->
coding sequence: tra genes
marker(s): cmlR
Cross references:

Insert

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Bacillus thuringiensis subsp. kurstaki
Gene name: crystal protein (delta endotoxin)
Contains complete coding sequence?: Y
Strain: HD-1
Type of DNA: plasmid
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 4.6
Cross references:

Insert lengths(kb): 4.599999904632568

Gene product: crystal protein (delta endotoxin)

Target Gene: crystal protein (delta endotoxin)

Insert Size (kb)

4.600

Media

ATCC^® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

The Tn5-B.t. gene segment (which conveys kanR) from pMAP29 was integrated into the host chromosome by mating E. coli HB101 containing pMAP29 with P. fluorescens 3732-3-7.

This strain is resistant to 100 mcg/ml rifampicin and 100 mcg/ml nalidixic acid.

Strain that proliferates in a plant environment to an extent sufficient to express at least 0.5 ng of insecticidally active protein per each insect to be controlled.

References

Obukowicz MG, et al. Combatting plant insect pests with plant-colonizing microorganisms containing the toxin gene B. thuringiensis as a chromosomal insertion. US Patent 5,229,112 dated Jul 20 1993

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

B231194：pMAP29 [112-12A, 3732-3-7A]

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