DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC19 Intact vector size: 2.686 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI HindIII Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI HindIII Construction: pUC71K Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ', <- MCS: EcoRI...HindIII, -> promoter: lac, <- replicon: pMB1, <- marker(s): ampR, <- Cross references: DNA Seq. Acc.: X02514
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CAST Gene name: calpastatin Contains complete coding sequence?: N Chromosome: 5; Localization: 5 q15-q21 Tissue: placenta Type of DNA: cDNA Insert end: Modification: EcoRI linker Insert end: Modification: EcoRI linker Insert size (kb): 1.837 Cross references: DNA Seq. Acc.: D16217 Nucleotides ~1-1594 of the insert correspond to nucleotides ~891-2486 of D16217. DNA Seq. Acc.: U31345 Nucleotides 1-1837 of the insert correspond to nucleotides 1-1837 of U31345.
Insert lengths(kb): 1.837000012397766
Tissue: placenta
Gene product: calpastatin [CAST]
Insert Size (kb)
1.837
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.8; BamHI--4.5; HindIII--2.8, 0.75, 0.55, 0.4; PstI--3.0, 1.3; EcoRI/PstI--2.7, 1.3, 0.6.
The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.68, 1.09, 1.66; PstI--1.20.