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GTpZapBglII

規格:

價格:詢價

編號:B177798

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

GTpZapBglII

商品貨號

B177798

Designations

GTpZapBglII

GenBank Number

M34353

培養基

Species

Homo sapiens, human

Depositors

MH Wigler

Vector

Construct size (kb): 6.599999904632568

Insert

DNA: cDNA

Insert lengths(kb): 3.619999885559082

Tissue: glioblastoma SW-1088 cell line

Gene product: avian UR2 sarcoma virus oncogene (v-ros) homolog [ROS]

Alleles: A1, A2, A1, A2

Insert Size (kb)

3.620

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

The insert includes the following restriction sites (nt positions according to the published sequence): BglII--4382; EcoRI--4623, 6183, 6649, 6845, 6965; HindIII--5438, 6939; BamHI--5631; PstI--5640; BglI--6333; AccI--6376, 6409.

Restriction digests of the clone give the following sizes (kb): EcoRI--4.3, 1.5, 0.5, 0.3: BamHI--2.9, 2.3, 1.3; HindIII--3.3, 1.5, 1.1, 0.6; PstI--5.2, 1.3; NotI--3.6, 2.9.

Extends from the BglII site at nt 4382 to approximately nt 8000.

The open reading frame extends from nt 207 to nt 7247. There is approximately 1 kb of 3' untranslated sequence which has not been fully characterized.

Subcloned from lambdagt10.165 which was constructed by adding NotI linkers to a blunt-ended BglII fragment.

References

Birchmeier C, et al. Characterization of ROS1 cDNA from a human glioblastoma cell line. Proc. Natl. Acad. Sci. USA 87: 4799-4802, 1990. PubMed: 2352949

Michael H Wigler, personal communication

B216188：GTpZapBglII

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